The central goal is to obtain pure full-length sacsin in high enough concentration for
structural and functional assays. We also aim to express and study the interaction of large
multidomain fragments of sacsin. These will allow us to study the structure of sacsin by X-ray
crystallography and cryo-electron microscopy. Purified protein will also enable assays of sacsin
chaperone function and protein-protein interactions.

Duration: one year
Grant: $80,000


Dr.Kalle Gehring Dept. of Biochemistry, McGill University
Life Science Complex, Room 469, 3649 Prom. Sir William Osler,Montreal QC, H3G 0B1
Tel: 514-398-7287;