{"id":10829,"date":"2010-03-20T17:54:05","date_gmt":"2010-03-20T17:54:05","guid":{"rendered":"https:\/\/clone.arsacs.com\/studies-of-transgenic-mice\/"},"modified":"2026-03-16T18:07:11","modified_gmt":"2026-03-16T22:07:11","slug":"studies-of-transgenic-mice","status":"publish","type":"post","link":"https:\/\/arsacs.com\/fr\/studies-of-transgenic-mice\/","title":{"rendered":"Studies of transgenic mice"},"content":{"rendered":"KO mice\n\n\u2022 Phenotype the SACS KO mice using the following instruments: Rotarod, Micro CT, Grip- strenght, Open-field test.\n\u2022 Continue the histological analysis of the SACS KO : DRG, muscles, retina, sciatic and optic nerves.\n\u2022 Test different neuronal modifications.\n\u2022 Build capacity for therapeutic trials on the mice. \n\n\n\nKI mice\n\nEstablish the colony of knock-in R272C mice.\n\n\u2022 Start clinical, histolocal, protein and electrophysiological analyses.\n\nOrganotypic models of the hyppocampus and cerebellum\n\n\u2022 Optimize organotypic cultures of hyppocampus and cerebellum from provenant sacsin KO mice.\n\u2022 Examine the anatomical changes and the electrical activity of the neurons in the organotypic cultures of the hyppocampus and the cerebellum of the KO mice.\n\u2022 Induce a knock-down using a virus to determine the effects on the functions and the neuronal development.\n\u2022 Arrange the transfer of transgenic mice with GFP in the membrane of the Purkinje cells to mate with the sacsin KO mice.\n\u2022 Establish the link at the anatomical and electrophysiological level between the sacsin and the Angelmann syndrome and more specifically the role of N+H+ NHE6 exchanger.\n\nPublications\n\n\u2022 Article under the supervision of Dr. Brais and describing the repertoire of mutations in the SACS gene in the Quebecois cases of ARSACS to be tabled in Fall 2010.\n\u2022 Article on the biology of the sacsin cell, under the supervision of Dr. McPherson, to be tabled in Fall 2010.\n\u2022 Article on the HEPN domain structure to be tabled by Dr. Gehring in Fall 2010.\n\u2022 Article on clinical and pathological characterization of the KO mice, under the supervision of Dr. Brais, to be tabled in Fall 2011. \n\nBroadcast\n\n\u2022 Submission of an abstract at the Keystone Symposia on Neurodegenerative Diseases : \"The Molecular and Cellular Basis for Neurodegenation\" in February 2011 ( Taos, Nouveau-Mexique).\n\u2022 Organize a meeting of researchers on ataxias at the American Society of Human Genetics Congress to be held in Montreal in Fall 2011. \n\nGranting Agencies\n\n\u2022 Apply in the Fall 2010 for a grant as part of the Institut g\u00e9n\u00e9tique des IRSC program and in partnership with the Foundation of Ataxia Charlevoix-Saguenay.","protected":false},"excerpt":{"rendered":"<p>KO mice \u2022 Phenotype the SACS KO mice using the following instruments: Rotarod, Micro CT, Grip- strenght, Open-field test. \u2022 Continue the histological analysis of the SACS KO : DRG, muscles, retina, sciatic and optic nerves. \u2022 Test different neuronal modifications. \u2022 Build capacity for therapeutic trials on the mice. KI mice Establish the colony [&#8230;]<\/p>\n","protected":false},"author":3,"featured_media":9248,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-10829","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-11"],"_links":{"self":[{"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/posts\/10829","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/comments?post=10829"}],"version-history":[{"count":1,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/posts\/10829\/revisions"}],"predecessor-version":[{"id":24075,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/posts\/10829\/revisions\/24075"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/media\/9248"}],"wp:attachment":[{"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/media?parent=10829"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/categories?post=10829"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/tags?post=10829"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}