{"id":11177,"date":"2018-07-27T21:43:36","date_gmt":"2018-07-27T21:43:36","guid":{"rendered":"https:\/\/clone.arsacs.com\/high-throughput-screening-of-small-molecule-libraries-to-identify-novel-therapeutics-for-arsacs-using-patient-cells-dr-paola-giunti-and-dr-suran-nethisinghe\/"},"modified":"2026-03-16T18:09:58","modified_gmt":"2026-03-16T22:09:58","slug":"high-throughput-screening-of-small-molecule-libraries-to-identify-novel-therapeutics-for-arsacs-using-patient-cells-dr-paola-giunti-and-dr-suran-nethisinghe-2","status":"publish","type":"post","link":"https:\/\/arsacs.com\/fr\/high-throughput-screening-of-small-molecule-libraries-to-identify-novel-therapeutics-for-arsacs-using-patient-cells-dr-paola-giunti-and-dr-suran-nethisinghe-2\/","title":{"rendered":"High-throughput Screening of Small Molecule Libraries to Identify Novel Therapeutics for ARSACS Using Patient Cells &#8211; Dr. Paola Giunti and Dr. Suran Nethisinghe"},"content":{"rendered":"This research consists of :\n1. Establishing baseline measurements for our mitochondrial and cytoskeletal phenotypic readouts on\nthe Perkin Elmer Opera Phenix High Content Screening platform. We will use\ntetramethylrhodamine methyl ester perchlorate (TMRM, a cationic fluorescent dye sequestered\nby healthy mitochondria) to measure basal mitochondrial membrane potential (\u0394Ym) as a read\nout of mitochondrial health in live cells. In parallel, fixed cells will be dual-stained by\nimmunofluorescence for Tom20 and vimentin to examine the mitochondrial network and\nintermediate filament morphology, respectively. We will set out and optimise the parameters for\nthe screen using ARSACS patient and control fibroblast lines.\n\n2. Performing small molecule library screens of Reata Pharmaceutical\u2019s compound library and\nBioAscent\u2019s Phenotypic Toolbox.\n\n3. Validating positive hits using traditional confocal, immunoblotting and luminescence assays. This\nwill include examining NADH levels and calcium signalling on a CCD camera (14, 15). Respiration\nrate and oxygen consumption will be measured with a Clark-type oxygen electrode (16). These\nstudies will provide mechanistic insight into the mode of action of these compounds.\n\nDuration: one year\nGrant: $50,000\n\nContacts\n\n<div id=\"attachment_277\" style=\"width: 130px\" class=\"wp-caption alignleft\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-277\" src=\"https:\/\/arsacs.com\/wp-content\/uploads\/2021\/02\/GiuntiPaola-120x150-2.jpg\" alt=\"Dr. Paola Giunti\" width=\"120\" height=\"150\" class=\"size-thumbnail wp-image-277\"><p id=\"caption-attachment-277\" class=\"wp-caption-text\">Dr. Paola Giunti<\/p><\/div>\n\nDr. Paola Giunti, Ataxia Centre, Department of Molecular Neuroscience\nUCL Institute of Neurology, Queen Square House\nQueen Square, London WC1N 3BG, United Kingdom\nTel: +44 (0)20 3448 3153;\n\n<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/arsacs.com\/wp-content\/uploads\/2021\/02\/Suran_Nethisinghe-150x150-2.jpg\" alt=\"\" width=\"150\" height=\"150\" class=\"alignleft size-thumbnail wp-image-3640\">\nDr. Suran Nethisinghe, Ataxia Centre, Department of Molecular Neuroscience\nUCL Institute of Neurology, Queen Square House\nQueen Square, London WC1N 3BG, United Kingdom\nTel: +44 (0)20 3448 3153; email: S.Nethisinghe@ucl.ac.uk","protected":false},"excerpt":{"rendered":"<p>This research consists of : 1. Establishing baseline measurements for our mitochondrial and cytoskeletal phenotypic readouts on the Perkin Elmer Opera Phenix High Content Screening platform. We will use tetramethylrhodamine methyl ester perchlorate (TMRM, a cationic fluorescent dye sequestered by healthy mitochondria) to measure basal mitochondrial membrane potential (\u0394Ym) as a read out of mitochondrial [&#8230;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[26],"tags":[],"class_list":["post-11177","post","type-post","status-publish","format-standard","hentry","category-research-2018"],"_links":{"self":[{"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/posts\/11177","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/comments?post=11177"}],"version-history":[{"count":1,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/posts\/11177\/revisions"}],"predecessor-version":[{"id":24405,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/posts\/11177\/revisions\/24405"}],"wp:attachment":[{"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/media?parent=11177"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/categories?post=11177"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/tags?post=11177"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}