{"id":11597,"date":"2016-04-02T22:03:55","date_gmt":"2016-04-02T22:03:55","guid":{"rendered":"https:\/\/clone.arsacs.com\/high-throughput-strategy-to-identify-large-domains-of-sacsin-dr-kalle-gehring\/"},"modified":"2016-04-02T22:03:55","modified_gmt":"2016-04-02T22:03:55","slug":"high-throughput-strategy-to-identify-large-domains-of-sacsin-dr-kalle-gehring","status":"publish","type":"post","link":"https:\/\/arsacs.com\/fr\/high-throughput-strategy-to-identify-large-domains-of-sacsin-dr-kalle-gehring\/","title":{"rendered":"“High-throughput strategy to identify large domains of Sacsin” \u2013 Dr Kalle Gehring"},"content":{"rendered":"

The greatest bottleneck in structural studies of Sacsin is the preparation and screening of fragments of thousands of Sacsin constructs with different boundaries, different mutations, and sequences from different species. To overcome this, we have developed a high-throughput strategy that relies on chemical DNA synthesis to prepare medium-sized libraries (mixtures) of Sacsin protein fragments. These are analyzed by gel filtration to isolate well-behaved proteins with solubility and stability characteristics that are favourable for structural studies. The proteins with these properties are then identified by mass spectrometry and studied by structural biology. The goal of the high–throughput strategy is to identify well-behaved Sacsin protein fragments for structural studies.<\/p>\n

Duration: one year
\nGrant: $98,280
\nContact:<\/p>\n

\"Dr

Dr Kalle Gehring<\/p><\/div>\n

Dr. Kalle Gehring, Professor Department of Biochemistry MCGill University
\nFrancesco Bellini Life Sciences Building, 3649 promenade Sir-William-Osler, Office: Room 469; Lab: Room 473, Montreal, Quebec H3G 0B1
\nTel:(514)-398-7287; Lab: (514)-398-2873\/1496; Fax:(514)-398-2983; Email: kalle.gehring@mcgill.ca<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

The greatest bottleneck in structural studies of Sacsin is the preparation and screening of fragments of thousands of Sacsin constructs with different boundaries, different mutations, and sequences from different species. To overcome this, we have developed a high-throughput strategy that relies on chemical DNA synthesis to prepare medium-sized libraries (mixtures) of Sacsin protein fragments. These […]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","inline_featured_image":false,"footnotes":""},"categories":[23],"tags":[],"class_list":["post-11597","post","type-post","status-publish","format-standard","hentry","category-recherche-2016"],"yoast_head":"\n"High-throughput strategy to identify large domains of Sacsin" \u2013 Dr Kalle Gehring - ARSACS<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/arsacs.com\/fr\/high-throughput-strategy-to-identify-large-domains-of-sacsin-dr-kalle-gehring\/\" \/>\n<meta property=\"og:locale\" content=\"fr_FR\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\""High-throughput strategy to identify large domains of Sacsin" \u2013 Dr Kalle Gehring - ARSACS\" \/>\n<meta property=\"og:description\" content=\"The greatest bottleneck in structural studies of Sacsin is the preparation and screening of fragments of thousands of Sacsin constructs with different boundaries, different mutations, and sequences from different species. 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