{"id":17978,"date":"2022-12-04T18:16:56","date_gmt":"2022-12-04T23:16:56","guid":{"rendered":"https:\/\/arsacs.com\/?p=17978"},"modified":"2026-03-16T18:14:44","modified_gmt":"2026-03-16T22:14:44","slug":"the-krogan-lab-team-continues-working-on-arsacs","status":"publish","type":"post","link":"https:\/\/arsacs.com\/fr\/the-krogan-lab-team-continues-working-on-arsacs\/","title":{"rendered":"The Krogan Lab team continues working on ARSACS"},"content":{"rendered":"<p class=\"wp-block-paragraph\">The Krogan Lab researchers are currently performing mechanistic follow-up studies on a subset of the protein-protein  interactions they &nbsp;identified. <br><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><br>\u201cOur affinity purification mass spectrometry studies on sacsin have revealed a protein-protein<br>interaction with DAPK (Death-associated protein kinase)1\/3, a positive mediator of gammainterferon<br>induced programmed cell death. Additionally, our analysis of the phosphorylation<br>landscape of WT and SACS-\/- cell lines followed by bioinformatics analysis revealed that tau, a<br>known DAPK1 substrate, is differentially phosphorylated. To understand the role of sacsin in<br>DAPK1-mediated tau phosphorylation, we are currently overexpressing or inhibiting DAPK1. In<br>addition, to identify which region of the multi-domain protein sacsin is required to regulate tau<br>phosphorylation, we are expressing individual domains separately followed by analysis of tau<br>phosphorylation\u201d.<br><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"has-text-align-left wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>","protected":false},"excerpt":{"rendered":"<p>The Krogan Lab researchers are currently performing mechanistic follow-up studies on a subset of the protein-protein interactions they &nbsp;identified. \u201cOur affinity purification mass spectrometry studies on sacsin have revealed a protein-proteininteraction with DAPK (Death-associated protein kinase)1\/3, a positive mediator of gammainterferoninduced programmed cell death. Additionally, our analysis of the phosphorylationlandscape of WT and SACS-\/- cell [&#8230;]<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[87],"tags":[],"class_list":["post-17978","post","type-post","status-publish","format-standard","hentry","category-recherche-2022"],"_links":{"self":[{"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/posts\/17978","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/comments?post=17978"}],"version-history":[{"count":5,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/posts\/17978\/revisions"}],"predecessor-version":[{"id":24852,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/posts\/17978\/revisions\/24852"}],"wp:attachment":[{"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/media?parent=17978"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/categories?post=17978"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/arsacs.com\/fr\/wp-json\/wp\/v2\/tags?post=17978"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}