The SACS protein, whose mutation results in ARSACS, is among the largest proteins in human cells (4579 residues), which hindered the determination of its structure and made basic biochemical experiments problematic. In the proposed project, we aim to use novel tagging approaches to allow SACS purification in its native state for structural determination, and also for its super-resolution localization in fixed and live cells.
Duration : one year
Dr. Walid A. Houry, Department of Biochemistry
Temerty Faculty of Medicine, University of Toronto
661 University Avenue,MaRS Centre, West Tower, Room 1612
Toronto, ON Canada M5G 1M1