The SACS protein, whose mutation results in ARSACS, is among the largest proteins in human cells (4579 residues), which hindered the determination of its structure and made basic biochemical experiments problematic. In the proposed project, we aim to use novel tagging approaches to allow SACS purification in its native state for structural determination, and also for its super-resolution localization in fixed and live cells.

Grant: $80,000

Duration : one year


Dr. Walid A. Houry, Department of Biochemistry

Temerty Faculty of Medicine, University of Toronto
661 University Avenue,MaRS Centre, West Tower, Room 1612

Toronto, ON Canada M5G 1M1