Functional and Structural Studies of Sacsin Using Baculovirus Expression Systems- Dr. Kalle Gehring

The central goal is to obtain pure full-length sacsin in high enough concentration for structural and functional assays. We also aim to express and study the interaction of large multidomain fragments of sacsin. These will allow us to study the structure of sacsin by X-ray crystallography and cryo-electron microscopy. Purified protein will also enable assays of sacsin chaperone function and protein-protein interactions. Duration: one year Grant: $80,000 Contact: Dr.Kalle Gehring Dept. of Biochemistry, McGill University Life Science Complex, Room 469, 3649 Prom. Sir William Osler,Montreal QC, H3G 0B1 Tel: 514-398-7287; Email:kalle.gehring@mcgill.ca